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The PCR initial activation step was performed at 95C for 15 min to activate the polymerase, followed by 40 three-step cycles of 15 min at 94C, 30 min at 55C and 30 min at 72C
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This appears to be unique to MGST1 as soluble GSTs and MPGES1 do not accept N-acetyl-L-cysteine as substrate
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