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By treating metabolically active adipocytes and hepatocytes with 5-amino-1-methylquinolinium, researchers can precisely inhibit intracellular NNMT activity, monitor changes in the nicotinamide pathway and NAD+ levels, and analyze effects on energy metabolism
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In the future, we will combine Western blot and immunofluorescence experiments to purposefully elucidate the molecular mechanisms of PI3K, p53, and other candidate pathways in the progression of RRM2 -mediated PTC, providing a more complete theoretical basis
Crossref Depoorter E, De Canck E, Coenye T, Vandamme P
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