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The uric acid was dissolved in the cell-growing medium without any supplements to reach the following concentrations: 200 M, 400 M, 500 M, 800 M, and 1000 M

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A lowering over time or oxidization of glutathione is natural

Healthy cells have a GSH/GSSG ratio above 100 at rest, while cells exposed to oxidative stress have a ratio between 1 and 10

Consequently, changes in the ROS levels inside the cancer cells in a 1% O 2 atmosphere were examined by utilizing the ROS specific probe 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) which is chemically converted into a green fluorescent dye in the action of ROS