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For mitochondrial activity, the cells were incubated with 200 mM MitoTracker Deep Red (Thermo Fisher Scientific, Barcelona, Spain) for 20 min at 38.5 C in the dark and then stained with 10 M YO-PRO-1 and 0.5 M PI

AOD-9604 has been shown to stimulate the breakdown of fat (lipolysis) and inhibit the formation of new fat (lipogenesis), particularly in areas with high fat concentration like the stomach and thighs [[R]

Under pathological conditions, liver macrophages can polarize into distinct phenotypes (M1 and M2), which play crucial roles in promoting inflammation and liver fibrosis

Slowing the infusion rate can help reduce this effect

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