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The synthesized cDNA was then subjected to RT-qPCR using the BeyoFast SYBR Green One-Step qRT-PCR Kit (D7268S, Beyotime) and the ABI PRISM 7500 RT-PCR system (Applied Biosystems, Thermo Fisher)
Slow Responders (15-20% of users): May not notice meaningful changes until week 3 or 4, with peak benefit reached around week 8 to 10
Clinico- experimental, prospective, sequential study]
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