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Metformin has a known GI effect that tends toward looser stools in some patients, which can partially offset GLP-1-related constipation

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Burr cells, also known as echinocytes (or hedgehog cells), are red blood cells with a spiky or serrated outer surface, giving them a thorny appearance that contrasts with the smooth, disc-shaped profile of healthy red blood cells

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Van Kampen-Zijlstras reagent was then added before measuring the absorbance of the supernatants at 540 nm using a microplate reader