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Immunoprecipitation (IP) and western blotting (WB) were performed as previously described 33
Although all the proteins of the PDHc have been found to be localized to the nucleus, the major protein of the PDHc that has been shown to be transported into the nucleus is the subunit of the E1 portion of the complex which is encoded by the PDHA1 gene
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In order to eliminate the potential baseline effect, the efficacy of l-carnitine, acetyl-l-carnitine, and propionyl-l-carnitine were evaluated using BMI change rate from the baseline value
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