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Avoid Repeated Freeze-Thaw If long-term storage of reconstituted solution is needed, aliquot into single-use volumes before freezing
Some call it, skinny shot, or lipofit
Representative tissue specimens were embedded in paraffin, sectioned at 4 m, stained with hematoxylin and eosin (H&E), and evaluated by light microscopy using an Olympus 71 digital camera and an Olympus BX51 microscope (Japan) acquiring digital images saved as uncompressed 24-bit RGB TIFF files
Araujo JA, Rivera Cd, Ethier JL, et al
After treatment, tissues were maintained in 2% FBS DMEM until the 10th days, the medium was changed every 3 days
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