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Indeed, the stronger relationship between elevated circulating homocysteine levels and atherothrombotic disease observed in retrospective case-controlled studies than in prospective studies might be explained by the younger average age of subjects sustaining a coronary event in retrospective studies (in the positive study by Wald et al (1998), see the age comparison between retrospective and prospective studies)
Yes, you can transition between routes, but dosing adjustments are necessary due to bioavailability differences
After the treatment, samples were precipitated with MeOH and after evaporation, obtained residue was dissolved in 50 L of 50 mM HEPES buffer, diluted with 20% ACN (containing 0.1% FA and mixture of internal standards) and processed for LC-MS/MS analysis
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