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Trypsin was neutralized with 500 l complete medium, the cells were resuspended and 100 l of the suspension was mixed with 6.9 ml Beckman Coulter Isoton II diluent (Fisher Scientific, NC2470899) for counting with a Beckman Coulter Z1 Coulter Cell Particle counter

al., Results of A Pulmonary Rehabilitation Program (PRP) In COPD Patients: Differences in Outcomes According to Currently 2011 GOLD Groups, European Respiratory Journal 44, no

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