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In this assay, the 30 min incubation duration was chosen to be optimal because of the inherently cytotoxic nature of CuCl 2 and Cu(OAc) 2 towards HaCaT keratinocytes, as they were observed to cause substantial cell death at a duration longer than 30 mins

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At low doses (such as 10-100 g/kg), the absorption efficiency is higher, and as the dosage increases, the absorption efficiency gradually saturates

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