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Furthermore, the specific inhibition of NNMT by 5-amino-1mq avoids interference with other metabolic enzymes, ensuring the specificity and reliability of experimental results

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Given that both diet composition and adiposity can each substantially impact the gut microflora in obese mice 24 , we conducted a secondary endpoint study using animals given the same series of diets to test the hypothesis that NNMTi treatment combined with LD, but not LD alone, shifts the cecal microbiome profile of obese animals to be similar to age-matched LD controls that were never given a high-fat diet