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The Vbeam laser treatment is quite comfortable, and though no topical anesthetic is required, it may be used

75 ug of proteins were reduced in 50 L of 10 mM HEPESKOH pH 7.5, 8 M urea containing Dithiothreitol (DTT) to a final concentration of 5 mM followed by heating at 95 C for 2 min, and a 30-min incubation at room temperature

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10.1038/ncomms1559 4 BannwarthB.NetterP.LapicqueF.GilletP.PrP.BoccardE.et al (1992)

Follow-up visits: 15-20 minutes monthly for the first few months, then every 6-8 weeks once stable