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During the acute phase of ischemic stroke, astrocytes undergo important morphological modifications and perform multiple functions both detrimental and beneficial for neuronal survival 59

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On the 6th day after seeding, 5 10 4 cells/well GBM cells were seeded in the 24-well plate, on the 7thy day the inserts with b.End3 cells were placed on top of the 24-well plate with the GBM cells, and experimental conditions were added

Even without a deficiency, they provide the structure and processes necessary throughout every stage of life