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Slices were then incubated for 10 min at 34 C (monitoring of bubbling, keeping slices in motion using a pipette) in wells containing 600 L of aCSF at 34 C (including 1 mM or 5 mM glucose) under fine bubble carbogen perfusion (1 Hz)
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Think of free radicals as tiny troublemakers that can damage your cells
Step therapy is a strategy where you try other less expensive medications first before Zepbound
If there is still medicine in the pens once it goes past these time frames, then it is important to dispose of the pens as the effectiveness would have been reduced
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