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Horseradish peroxidase (HRP)-conjugated anti-rabbit goat antibody was purchased from Dako

Dopaminergic Reward Circuit Enhancement: Melanocortin receptor activation influences mesolimbic dopamine pathways projecting from the ventral tegmental area to the nucleus accumbens

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Activation of the receptor leads to stimulation of the Gq/11 protein-dependent pathway, an increase in IP3 concentration, the release of calcium ions from the endoplasmic reticulum and, consequently, the exocytosis of GH-containing granules