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The lysed tissue was centrifuged (13000 rpm, 3 min, 4 C) and the supernatant was transferred to a clean Eppendorf tube (low-bind protein) prior to protein concentration determination with the DC Protein Assay (Bio-Rad) according to manufacturers instruction
If there is a lack of cobalamin in the body, methylmalonyl CoA accumulates, which presumably leads to the neurological manifestations of B12 deficiency
Patients were randomized to apply glutathione as 2% GSSG lotion and a placebo lotion in a split-face protocol, twice daily for ten weeks
remains investigational, not approved
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