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Following this, Banting signed a contract with Connaught Laboratories of the University of Toronto for the large-scale production of their insulin extract which they used to successfully treat a further six patients
The Lancet HIV, 7(7), e401e409
Quantitative real-time polymerase chain reaction (PCR) analysis Total RNA was extracted using a TRIzol kit (Invitrogen, Carlsbad, CA, USA) and cDNA synthesis was performed using a cDNA synthesis kit (Fermentas Life Sciences) according to the manufacturer instructions
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