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The culture was harvested by centrifugation following a 16-h incubation and resuspended in lysis buffer (50 mM Tris-HCl pH 8.0, 300 mM NaCl, 10% glycerol, 10 mM imidazole and 1 mM DTT containing cOmplete EDTA-free protease inhibitor (Roche))

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In patients with CVD who seek pharmacological treatment for weight loss, Endocrine Society Guidelines suggest using medications that are not sympathomimetics, such as orlistat

Interaction between hormone-sensitive lipase and ChREBP in fat cells controls insulin sensitivity

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