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Cell pellets were collected and resuspended in lysis buffer (50 mM Tris pH 8.0, 200 mM NaCl, 5 mM -mercaptoethanol, 10% glycerol, and 1X Halt protease inhibitor (Thermo Fisher), and frozen overnight at 80 C

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Data and model availability The experimental data as well as the complete code for data analysis and modelling are available at (DOI: 10.5281/zenodo.5070401) and is mirrored at Author Contributions All authors were involved in the writing of the paper

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Nothing is opened, mixed or transferred by hand along the way