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To image endocytosis of ecto-Halo 1 in live cells, cells were labeled with 250 nM Alexa488 Halo ligand for 5 min, washed twice with medium at RT, and imaged live at 37 C with a TIRFM microscope and HILO illumination

In practical terms, both compounds currently sit in a transitional regulatory status: no longer explicitly restricted, but not yet authorized for routine compounding either

Proc Natl Acad Sci U S A 99:350510 Brown GC, Borutaite V (2004) Inhibition of mitochondrial respiratory complex I by nitric oxide, peroxynitrite and S -nitrosothiols

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Potential issues and how to avoid them Combining potent actives requires awareness of possible problems