[8] Each individual B vitamin is water-soluble and generally very safe at physiologic doses, but the combination product should only be used when clearly needed
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For all subsequent analyses, samples were resuspended in 5% sodium dodecyl sulfate (SDS) 50 mM triethylammonium bicarbonate (TEAB), enough to cover the pellet, placed in 95 C for 10 min and thereafter clarified by centrifuging at 10,000 g for 10 min at room temperature