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To reduce lipofuscin autofluorescence, sections were further treated with TrueBlack Lipofuscin Autofluorescence Quencher (Biotium, Inc., Fremont, CA, USA), diluted with 70% ethanol for 30 s, and briefly washed with PBS before mounting with VECTASHIELD mounting medium (Vector laboratories, Newark, CA, USA) and assessed using LSM880 with the Airyscan laser scanning microscope system (Carl Zeiss)
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