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To extend our investigation, we employed the hydrodynamic tail-vein injection (HDTVi) model with spontaneous HCC tumors induced by CRISPR/Cas9-mediated loss-of-function genome editing of endogenous TP53 and Sleeping Beauty (SB) transposon-driven c-Myc overexpression in C57BL/6 mice [16] (Additional file 1: Fig

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