In planta biosensor measurements The multiparametric real-time measurements were conducted with a CLARIOstar multiwell plate-reader equipped with an atmospheric control unit (BMG Labtech, Ortenberg, Germany)
Managing B12 Deficiency and Optimization Supplementation Strategies Effective B12 supplementation requires consideration of individual absorption capacity, deficiency severity, and underlying health conditions
Dihexa is not a conventional peptide but a peptidomimetic a small molecule (~504 Da) designed to mimic peptide binding activity while resisting enzymatic degradation
Antibodies used include MAP2 for neurons (I3-1500) (1:200), SOX2 for neural progenitors (MAB2018) (1:200), GFAP for astrocytes (Abcam, Cambridge, UK, #ab4674) (1:500) and ASC for ASC specks (AL177) (1:200) to assess the NLRP3 inflammasome assembly and ASC specks formation in astrocytes
Arslan F, Lai RC, Smeets MB et al (2013) Mesenchymal stem cell-derived exosomes increase ATP levels, decrease oxidative stress and activate PI3K/Akt pathway to enhance myocardial viability and prevent adverse remodeling after myocardial ischemia/reperfusion injury