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These results indicate that many mutations acquired during early rounds of PANCE and dual selection were not essential for gasdermin D cleavage and, in some cases, were detrimental to protease efficiency in the context of GD_L variants
10.1186/s12906-016-1072-6 63 GhazaleeN
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In prolotherapy, because an inflammatory agent is used, there is a chance of a flare-up of pain and swelling post-injection
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