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Then, cells were incubated overnight at 4C by using primary antibodies LC3A/B (1:1000 dilution, #4108, Cell Signaling Technology, Massachusetts, United States), p62 (1:2000 dilution, ab56416, Abcam, Massachusetts, United States), PINK1 (1:1000 dilution, 23274-1-AP, Proteintech Group, Illinois, United States), and Parkin (1:1000 dilution, ab15954, Abcam, Massachusetts, United States)

#Reference: Hribar, M., Goljuf, ., & Pravst, I

Within this signature, caspase-1 emerged as a top-tier biomarker of protease sensitivity (log 2 (fold change) = 1.28, P = 0.024

The answer isnt as clear cut as you might imagine, suggests endocrinologist Caroline Messer, M.D

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