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Imaging was performed 24 hours post-transfection using excitation wavelengths of 405 nm and 488 nm, allowing calculation of the redox state under basal conditions, after oxidation with hydrogen peroxide (1 mM H2O2), and following reduction with dithiothreitol (5 mM DTT)
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She adds that when our mitochondria arent working well, it can contribute to fatigue and mood issues
ELISA Following treatment, conditioned medium was recovered and used to determine concentration of IL-1, IL8, HSPA1A and FOSL1
