centrifuge at 10,000 g for 10-15 min at 4C, and remove the supernatant for subsequent reduction of glutathione assay
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Following GM dysbiosis, abnormalities in bacterial products and metabolites, such as choline, bile acids(BAs), short-chain fatty acids (SCFAs), lipopolysaccharide (LPS), and Toll-like receptor 4 (TLR4), also occur, further hastening the onset and progression of NAFLD [32]
Fibroblasts are connective tissue cells responsible for producing collagen and the extracellular matrix that gives tissue its structure