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Cell culture and transfection WT HepG2 or stably transfected cells (pSuperior.puro-GSTM2) were cultured in DMEM (HyClone, Logan, UT, USA) containing 10% foetal bovine serum (Clark Bioscience, Richmond, VA, USA), 100 U/mL penicillin, and 100 g/mL streptomycin in dishes at 37 C and transfected with Lipo6000 Transfection Reagent (#C0528, Beyotime)
Int J Mol Sci 22(6)
Signs You Need Professional Treatment for Black Patches on Inner Thighs Look out for these signs to know when to see a doctor: Persistent dark patches that dont get better with home treatments Darkness or patches spreading Itching, redness, or irritation Not sure why the dark patches are there Professional treatment can give a proper diagnosis and targeted therapy
However, many factorssuch as aging, stress, poor diet, and lifestyle habitscan lead to low testosterone levels
In particular, LH stimulates high concentrations of testosterone (ITT) in the testis, which is essential for spermatogenesis
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