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before all other reagents were added, and absorbance at 340 nm was measured over time
p-eIF4E staining was done using mouse anti-elF4E (pS209) (BD 560229) diluted 1:100 and cells were fixed with BD Cytofix/Perm and intracellular staining was done in BD Perm (BD 554714)
Results are very patient-dependent, so we cant guarantee a specific outcome
This means it can lose efficacy when exposed to sunlight without the help of a stabilizing agent
We monitor patients and adjust protocols based on response
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