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Individual hair follicles were isolated and cultured in Williams Medium E (Sigma-Aldrich), supplemented with 2 mM L-glutamine (Sigma-Aldrich), 10 g/ml insulin (Sigma-Aldrich), 100 ng/ml hydrocortisone (Sigma-Aldrich), 0.1% fungizone (Gibco), 1% antibiotic-antimycotic (Gibco), and 1% Penicillin-Streptavidin (Gibco) under standard conditions (37 C, 5% CO2)
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The FASEB Journal
The TB-500 weekly band rests on two veterinary anchors that disagree by 2.6x an equine protocol scaling to 2.29 mg/week for a 70 kg human, and back-calculation from a 5 mg greyhound dose giving 5.91 mg/week
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