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The isolated cDNAs from the pool enriched in the fourth and fifth rounds were ligated into the pGEM-T-Easy vector (Promega), using DNA Ligation Kit Mighty Mix (Takara Bio), and cloned into DH5 competent cells
For systemic benefits, tissue repair , or hair restoration , injectable protocols typically produce superior results due to better bioavailability and distribution
These reactions are less common than mild local reactions but may require immediate medical attention
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