The presence of leukocytes in the assay could interfere with parasite viability through phagocytosis or other immune mechanism and limit infection of the mosquitoes, and this, therefore, masks the effect of MB
Importantly, although RETA-w/d restored the tumor transcriptome close to that of Veh, the tumor progression remained relatively blunted, which could be due to factors external to the tumor including systemic metabolic effects, such as insulin concentration, which were only partially restored after RETA-w/d, as discussed above
Leukemia 15 (10), 16331640
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Guideline recommendations should be followed as the best synthesis of evidence and experts consensus, and expectations regarding effect size should remain appropriately tempered