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In biochemical and cellular research systems, DSIP has been examined for its involvement in neuroendocrine signalling, stress-associated pathways, and peptidereceptor interactions

Reagent 2 working solution preparation: Dissolve powder A (Reagent 2-powder A) completely in 170ml hot double distilled water at 90~100 to make solution A (Reagent 2 - Solution A), mix solution A (Reagent 2 - Solution A) and solution B (Reagent 2 - Solution B) sufficiently

Seahorse assay HT-1080 cells were seeded in Seahorse XFp six-well miniplates at the following densities

The degradation rate and half-life (t1/2) of the drugs were determined by measuring the decrease in area under the curve (AUC) at their retention times [1]

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