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1986 Jun;24(6):693-8

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This biosynthesis perfectly mimics how green tea leaves naturally produce theanine, guaranteeing a 100% pure L-isomer product entirely free of the competing D-isomer

To adjust the pH, 2 L of Tris-HCl pH 7.5 was added along with 12.5 L of premixed NuPAGE LDS Sample Buffer (Thermo Fisher Scientific #NP0007), and the samples were stored at 20 C