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After FASP digestion, samples were brought up in 2 % acetonitrile, 0.1 % formic acid and separated with an EasyLC into a QE-Plus (Thermo Fisher Scientific) mass spectrometer and eluted over a 90-min gradient from 100% buffer A (2% acetonitrile, 0.1% formic acid) to 100% buffer B (90% acetonitrile, 0.1% formic acid) with an MS resolution of 70,000 and an MS2 resolution of 35,000 running a top 15 DDA method
Correlated with this decrease in GSH levels, we found that the T2DM group also has significantly higher levels of MDA ( Figures 3B, C ) and higher levels of the proinflammatory cytokine IL-6 ( Figure 3D ) than the healthy group
On day 7 seedlings were transferred to fresh growth medium, and then some of the plates were supplemented with AITC to a final concentration of 1 mM whereas others remained non-treated (control)
Quality and purity risk: Both compounds are available only through unregulated research chemical markets
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