The approach remains theoretical but aligns with general principles of supporting recovery during periods of elevated repair activity
Then cDNA was synthesized using SuperScript TM IV VILO TM Master Mix (Invitrogen, Waltham, MA, United States) and diluted 101 by DNase/RNase-Free Distilled Water (Invitrogen)
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However, as the concept of ferroptosis had yet to be established, a definitive connection could not be drawn ( Trypanosoma cruzi tryparedoxin peroxidase results in lethal iron-dependent lipid peroxidation, leading to ferroptosis, with mitochondrial iron playing a pivotal role