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21 Statistical analysis All data were expressed as meanS.E.M

In addition, HA and FLAG expression tags were added to both 5 and 3 ends, respectively

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GSH/GSSG-Glo Assay A Luminescent-Based Assay to Detect and Quantify Total Glutathione Ratios Measures physiologically relevant GSH/GSSG ratios Simple protocol minimizes sample handling, reducing variability Stable assay signal amenable to high throughput analysis Catalog Number: Size Catalog Number: V6611 Catalog Number: V6612 Valuable Measurement of Glutathione (GSH-to-GSSG) Ratios The GSH/GSSG-Glo Assay is a luminescence-based system that detects and quantifies total glutathione (GSH + GSSG), GSSG and GSH-to-GSSG ratios in cultured cells

Kidney function should be checked before starting as it naturally declines with age