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Statistical significance of the differences in metabolite abundance between the MH and HpSC groups were quantified using a MannWhitney U test in the limma R package
Bile acids are synthesized from cholesterol in hepatocytes by various liver enzymes, including cholesterol 7-hydroxylase (CYP7A1), sterol 12-hydroxylase, and sterol-27-hydroxylase (CYP27A1) 39
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Glutathione is the strongest antioxidant our body can produce and is often called the master antioxidant
