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Cells were lysed in 1 mL of lysis buffer (10 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.5 mM EDTA, 1% NP40, 0.5% Triton X-100, and 10% glycerol) supplemented with 20 M PR619, 10 mM iodoacetamide, and a protease inhibitor cocktail and were sonicated using Handy Sonic (TOMY Seiko)
Fan S, Goldfarb E, Lacadie C, et
Peptides Commonly Discussed for Knee Inflammation and Recovery Several peptides come up repeatedly when knee patients research recovery options
In: Pollock D
& Helmy, W
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