At 0, 3, 7, and 10 days, the tissues were fixed, and cryosections were used for in situ apoptotic staining with terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL), according to the manufacturers instructions (Servicebio, Beijing, China)
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Thats why experienced clinicians dont begin by asking, Which peptide should we use? They begin by asking, What are we treating? That distinction influences every decision that follows, including whether peptide therapy is appropriate at all