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Cytosolic acetoacetate is converted to acetoacetyl-CoA which is then hydrolyzed into two moles of acetyl-CoA as described in the Nonoxidative Metabolic Fates of Ketones section below
This is due to the possibility that more glycine and serine may be needed to properly catabolize the extra methionine, unintentionally leading to a shortage in these amino acids
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Another source of deficiency can be malabsorption issues, where the body is unable to properly absorb B12 from the intestines due to various gastrointestinal disorders
The inclusion of benzyl alcohol helps reduce contamination risk during this time, supporting safe and efficient laboratory practices
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