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(4) The researchers reported, On days 6, 10, and 13, tripeptide-copper complex-treated wounds contained significantly lower concentrations of TNF-alpha and MMP-2 and MMP-9 than control wounds. Tumor necrosis factor-alpha (TNF-alpha), a cytokine involved in systemic inflammation, along with matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) enzymes that break down extracellular matrix proteins might potentially influence both the inflammatory processes and the remodeling of tissues

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The culture was then centrifuged at 6,000 RPM for 15 min, and the supernatant was discarded

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PMID 19320537