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In a retrospective matched cohort study using data from a centralized data registry of patients evaluated by neuro-ophthalmologists at 1 academic institution from December 1, 2017, through November 30, 2023, a search for International Statistical Classification of Diseases and Related Health Problems, Tenth Revision code H47.01 (ischemic optic neuropathy) and text search yielded 16 827 patients with no history of NAION

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Specifically, 120 mg of frozen floret powder was resuspended with 1.5 mL of pre-chilled 80% ( v / v ) acetone, vortexed for 1 min, and then clarified by centrifugation at 5,000 g for 5 min