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For immunostaining, cells were permeabilized for 1 min using ice-cold PBS supplemented with 0.1% Triton X-100, blocked for 30 min with PBS supplemented with 1% BSA, incubated with the primary antibody in blocking buffer for 2 h at room temperature (RT), washed and subsequently incubated with secondary antibodies and DAPI in blocking buffer for 2 h at RT

It is not approved for human or veterinary use and no therapeutic claims are made

This combinatorial coding capacity underscores the adaptability of the UPS and its capacity to orchestrate diverse cellular outcomes beyond protein destruction

Unlike model microorganisms such as E

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