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the gel purified His6-AV-Ubiquitin-semapeptide (nucleotide sequence) encoding gene PCR product and pET 24 plasmid vector was restriction digested (double digestion) with Ndel (NEB) + Xhol (NEB) at 37 C
After washing three times in PBS-PVA, the oocytes were mounted on slides under coverslips
to reduce the risk of major adverse cardiovascular (CV) events (CV death, non-fatal myocardial infarction or non-fatal stroke) in adults with type 2 diabetes mellitus who are at high risk for these events
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