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GHK-Cu (Glycyl-L-histidyl-L-lysine-Cu2+) is a small, naturally occurring copper-binding peptide that plays a vital role in tissue remodeling, inflammation reduction, and cellular communication

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Receptor binding patterns, intracellular transport mechanisms, and potential off-target effects remain incompletely characterized

step-a Gln(Trt)-AIa-AIa-Lys(C18diacidmono-t-butyI-y-Glu(AEEA-AEEA)-OtBu)-Glu (OtBu)-Phe-IIe-AIa-Trp(Boc)-Leu-VaI-Arg(pbf)-Gly-Arg(pbf)-Gly-OtBu (Fragment-6) was condensed with Fmoc-Ser(tBu)-Tyr(tBu)-Leu-Glu(OtBu)-Gly-OH (Fragment-3) in presence of coupling agent to obtain Fmoc-Ser(tBu)-Tyr(tBu)-Leu-Glu(OtBu)-Gly-Gln(Trt)-AIa-AIa-Lys (C18diacidmono-t-butyI-Y-Glu(AEEA-AEEA)-OtBu the coupling agent used in the reaction can be selected from group consisting of [Ethylcyano (hydroxyimino)acetate)-tri-(l-pyrrolidinyl)-Phosphonium hexa fluorophosphate (PyOxim), ethyl-2 -cyano-2-(hydroxy amino) acetate (Oxyma pure), 0-(benzotriazol-l-yl)-N,N,N,N'- tetramethyluronium tetrafluoroborate (TBTU), diisopropyl carbodiimide (DIC), 1,3- dicyclohexylcabodiimide (DCC) , 0-(7 -azabenzotriazol- 1 -yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate (HATU), 1 -(dimethyl aminopropyl)-3-ethylcarbodiimide hydrochloride (EDC the reaction temperature may range from 25 C to 30 C