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2.2 Identification of differentially expressed genes To identify differentially expressed genes related to GLP-1 signaling, data of tumor samples (from TCGA database) and normal tissue samples (from TCGA and GTEx databases) were analyzed using the limma R package, with the cut-off value of |log2-fold change| 1 and FDR T, accounting for more than 50% and most of the gene mutations were missense mutations (Figure 2A)