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dsDNA (calf thymus DNA-sodium salt Type 1 fibers, Sigma-Aldrich, Australia) and GSH stock solutions were prepared in sterile MilliQ water
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Although direct neo-N-terminal fragments of caspase-1 were not enriched in the TMT-TAILS dataset, potentially due to labeling inefficiencies, we observed significant depletion of peptides spanning the X(PC) cleavage site in the active protease-treated samples, providing strong evidence of on-target cleavage (Extended Data Fig
The key difference is dosing range
